Western Blot:Article Title: <p>INPP4B inhibits cell proliferation, invasion and chemoresistance in human hepatocellular carcinoma</p>
Article Snippet: .. Western blot analysis Western blots were performed using anti-INPP4B (Abcam, UK), anti-E-cadherin (Abcam, UK), anti-N-cadherin (Abcam, UK), anti-twist (OriGene, USA), anti-slug (OriGene, USA), anti-Cleaved caspase-3 (Cell Signaling Technology, USA), anti-Cleaved PARP (Cell Signaling Technology, USA), anti-Phospho-Akt (Invitrogen, USA), anti-Akt (Invitrogen, USA), anti-Phospho-PI3K (SigmaAldrich, USA), anti-PI3K (OriGene, USA), anti-p53 (Invitrogen, USA) and anti-GAPDH (Immunology Consultants Laboratory, USA) as previously described.27 Immunohistochemical assay Paired paraformaldehyde-fixed paraffin tissue chips were subjected to immunohistochemistry (IHC) assay, and staining evaluation was performed as previously reported.27,28 Briefly, staining signal intensities according to the chromatosis intensity, no staining, light yellow, buffy and brown are scored 0, 1, 2 and 3, respectively. ..
Immunohistochemical staining:Article Title: <p>INPP4B inhibits cell proliferation, invasion and chemoresistance in human hepatocellular carcinoma</p>
Article Snippet: .. Western blot analysis Western blots were performed using anti-INPP4B (Abcam, UK), anti-E-cadherin (Abcam, UK), anti-N-cadherin (Abcam, UK), anti-twist (OriGene, USA), anti-slug (OriGene, USA), anti-Cleaved caspase-3 (Cell Signaling Technology, USA), anti-Cleaved PARP (Cell Signaling Technology, USA), anti-Phospho-Akt (Invitrogen, USA), anti-Akt (Invitrogen, USA), anti-Phospho-PI3K (SigmaAldrich, USA), anti-PI3K (OriGene, USA), anti-p53 (Invitrogen, USA) and anti-GAPDH (Immunology Consultants Laboratory, USA) as previously described.27 Immunohistochemical assay Paired paraformaldehyde-fixed paraffin tissue chips were subjected to immunohistochemistry (IHC) assay, and staining evaluation was performed as previously reported.27,28 Briefly, staining signal intensities according to the chromatosis intensity, no staining, light yellow, buffy and brown are scored 0, 1, 2 and 3, respectively. ..
Immunohistochemistry:Article Title: <p>INPP4B inhibits cell proliferation, invasion and chemoresistance in human hepatocellular carcinoma</p>
Article Snippet: .. Western blot analysis Western blots were performed using anti-INPP4B (Abcam, UK), anti-E-cadherin (Abcam, UK), anti-N-cadherin (Abcam, UK), anti-twist (OriGene, USA), anti-slug (OriGene, USA), anti-Cleaved caspase-3 (Cell Signaling Technology, USA), anti-Cleaved PARP (Cell Signaling Technology, USA), anti-Phospho-Akt (Invitrogen, USA), anti-Akt (Invitrogen, USA), anti-Phospho-PI3K (SigmaAldrich, USA), anti-PI3K (OriGene, USA), anti-p53 (Invitrogen, USA) and anti-GAPDH (Immunology Consultants Laboratory, USA) as previously described.27 Immunohistochemical assay Paired paraformaldehyde-fixed paraffin tissue chips were subjected to immunohistochemistry (IHC) assay, and staining evaluation was performed as previously reported.27,28 Briefly, staining signal intensities according to the chromatosis intensity, no staining, light yellow, buffy and brown are scored 0, 1, 2 and 3, respectively. ..
Staining:Article Title: <p>INPP4B inhibits cell proliferation, invasion and chemoresistance in human hepatocellular carcinoma</p>
Article Snippet: .. Western blot analysis Western blots were performed using anti-INPP4B (Abcam, UK), anti-E-cadherin (Abcam, UK), anti-N-cadherin (Abcam, UK), anti-twist (OriGene, USA), anti-slug (OriGene, USA), anti-Cleaved caspase-3 (Cell Signaling Technology, USA), anti-Cleaved PARP (Cell Signaling Technology, USA), anti-Phospho-Akt (Invitrogen, USA), anti-Akt (Invitrogen, USA), anti-Phospho-PI3K (SigmaAldrich, USA), anti-PI3K (OriGene, USA), anti-p53 (Invitrogen, USA) and anti-GAPDH (Immunology Consultants Laboratory, USA) as previously described.27 Immunohistochemical assay Paired paraformaldehyde-fixed paraffin tissue chips were subjected to immunohistochemistry (IHC) assay, and staining evaluation was performed as previously reported.27,28 Briefly, staining signal intensities according to the chromatosis intensity, no staining, light yellow, buffy and brown are scored 0, 1, 2 and 3, respectively. ..
other:
Saline:Article Title: Estrogen receptor β regulates AKT activity through up-regulation of INPP4B and inhibits migration of prostate cancer cell line PC-3
Article Snippet: .. Membranes were blocked with 5% nonfat powdered milk in 0.1% Tris-buffered saline buffer and probed with anti-ERβ (PPZ0506, Invitrogen), anti-INPP4B (D9K1B), pAKT Thr308 (D25E6), pAKT Ser473 (D9E), panAKT (C67E7), pGSK3β (D85E12), (Cell Signaling Technology), GAPDH-HRP (sc-47724) (Santa Cruz Biotechnology), and β-Actin ( {"type":"entrez-protein","attrs":{"text":"A19780","term_id":"89794"}} A19780 ) (Millipore Sigma). ..
Incubation:Article Title: Collateral metabolic vulnerabilities unveiled by loss of isozyme diversity in breast cancer
Article Snippet: Subsequently, proteins were resolved by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were incubated with the specific primary antibodies, including anti-ACACB (1:1000, CST, rabbit monoclonal, cat. no. 8578), anti-ACACA (1:2000, Proteintech, rabbit polyclonal, cat. no. 21923-1-AP), anti-HMGCS2 (1:1000, CST, rabbit monoclonal, cat. no. 20940), anti-HMGCS1 (1:1000, Proteintech, rabbit polyclonal, cat. no. 17643-1-AP), anti-INPP4B (1:1000, CST, rabbit monoclonal, cat. no. #14543), anti-INPP4A (1:1000, Proteintech, rabbit polyclonal, cat. no. 12665-1-AP), anti-NMT2 (1:500, Santa Cruz, mouse monoclonal, cat. no. sc-136005), anti-NMT1 (1:2000, Proteintech, rabbit polyclonal, cat. no. 11546-1-AP), anti-Flag (1:1000, Sigma-Aldrich, mouse monoclonal, cat. no. F1804), anti-FXR2 (1:500, Santa Cruz, mouse monoclonal, cat. no. sc-32266), anti-vinculin (1:100000, ABclonal, rabbit monoclonal, cat. no. A2752), anti-GAPDH (1:20000, Proteintech, mouse monoclonal, cat. no. 60004-1-lg), anti-ACTB (1:20000, Proteintech, mouse monoclonal, cat. no. 66009-1-Ig). .. Secondary antibody conjugated with HRP (1:10000, goat anti-rabbit or goat ani-mouse, Proteintech) and enhanced chemiluminescence (Tanon) were employed to detect specific protein signals.
Article Title: Collateral metabolic vulnerabilities unveiled by loss of isozyme diversity in breast cancer.
Article Snippet: Subsequently, proteins were resolved by SDS-PAGE and transferred to polyvinylidene difluoride membranes (Millipore). .. The membranes were incubated with the specific primary antibodies, including anti-ACACB (1:1000, CST, rabbit monoclonal, cat no. 8578), anti-ACACA (1:2000, Proteintech, rabbit polyclonal, cat no. 21923-1-AP), anti-HMGCS2 (1:1000, CST, rabbit monoclonal, cat no. 20940), anti- HMGCS1 (1:1000, Proteintech, rabbit polyclonal, cat no. 17643-1-AP), anti-INPP4B (1:1000, CST, rabbit monoclonal, cat no. #14543), anti-INPP4A (1:1000, Proteintech, rabbit polyclonal, cat no. 12665-1-AP), anti-NMT2 (1:500, Santa Cruz, mouse monoclonal, cat no. sc-136005), anti-NMT1 (1:2000, Proteintech, rabbit polyclonal, cat no. 11546-1-AP), anti-Flag (1:1000, Sigma-Aldrich, mouse monoclonal, cat no. F1804), anti-FXR2 (1:500, Santa Cruz, mouse monoclonal, cat no. sc-32266), anti- vinculin (1:100000, ABclonal, rabbit monoclonal, cat no. A2752), anti-GAPDH AR TIC LE IN PR ES S (1:20000, Proteintech, mouse monoclonal, cat no. 60004-1-lg), anti-ACTB (1:20000, Proteintech, mouse monoclonal, 66009-1-Ig). .. Secondary antibody conjugated with HRP (1:10000, Goat anti-Rabbit or Goat ani-mouse, Proteintech) and enhanced chemiluminescent (Tanon) were employed to detect specific protein signals.
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